Frontiers in Veterinary Science (November 2022)
by Enrique Gómez; Antonio Murillo; Susana Carrocera; David Martín-González; Isabel Gimeno (Servicio Regional de Investigación y Desarrollo Agroalimentario – SERIDA); Juan José Pérez-Jánez (Cooperativa de Agricultores y Usuarios de Gijón); Jose Luis Benedito (University of Santiago de Compostela)
In cattle, vitrified/warmed (V/W) and frozen/thawed (F/T), in vitro-produced (IVP) embryos, dier in their physiology and survival from fresh embryos. In this study, we analyzed the eects of embryo cryopreservation techniques on the ospring. IVP embryos cultured with albumin and with or without 0.1% serum until Day 6, and thereafter in single culture without protein, were transferred to recipients on Day 7 as F/T, V/W, or fresh, resulting in N = 24, 14, and 13 calves, respectively. Calves were clinically examined at birth, and blood was analyzed before and after colostrum intake (Day 0), and subsequently on Day 15 and Day 30. On Day 0, calves from V/W and F/T embryos showed increased creatinine and capillary refill time (CRT) and reduced heartbeats. Calves from F/T embryos showed lower PCO2, hemoglobin, and packed cell volume than calves from V/W embryos while V/W embryos led to calves with increased Na+ levels. Colostrum eects did not dier between calves from fresh and cryopreserved embryos, indicating similar adaptive ability among calves. However, PCO2 did not decrease in calves from V/W embryos after colostrum intake. Serum in culture led to calves with aected (P < 0.05) temperature, CRT, HCO− 3 , base excess (BE), TCO2, creatinine, urea, and anion gap. On Day 15, the eects of embryo cryopreservation disappeared among calves. In contrast, Day 30 values were influenced by diarrhea appearance, mainly in calves from V/W embryos (i.e., lower values of TCO2, HCO− 3 , and BE; and increased glucose, anion gap, and lactate), although with no more clinical compromise than calves from fresh and F/T embryos. Diarrhea aected PCO2 and Na+ in all groups. Embryo cryopreservation, and/or culture, yield metabolically dierent calves, including eects on protein and acid–base metabolism.